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JoVE Journal Bioengineering
Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering
 

Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering

Article DOI: 10.3791/63622-v • 10:17 min • May 16th, 2022
May 16th, 2022 •
1,2,4, 3,4,5, 2,4,6, 2, 2,5, 4, 2,7, 2,7, 3,4,5,8
1Vocational School of Health Services, Istinye University, 2Chemical Metallurgy Faculty, Yildiz Technical University, 3Faculty of Medicine, Department of Histology & Embryology, Istinye University, 43D Bioprinting Design & Prototyping R&D Center, Istinye University, 5Stem Cell, and Tissue Engineering R&D Center, Istinye University, 6Vocational School of Health Services, Altinbas University, 7The V. Akhundov National Scientific Research Medical Prophylactic Institute, 8Center for Stem Cells and Regenerative Medicine (LivMedCell), Liv Hospital

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In this manuscript, we demonstrate the preparation of a biohybrid hydrogel bioink containing graphene for use in peripheral tissue engineering. Using this 3D biohybrid material, the neural differentiation protocol of stem cells is performed. This can be an important step in bringing similar biomaterials to the clinic.

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Graphene-based Biohybrid Hydrogel Bioink Peripheral Neuroengineering Stem Cell System Stem-cell Niche Peripheral Neuropathy 3D Culture Methods Spheroid Formation 3D Bioprinting Alginate/gelatin-based Bioinks Regenerative Features Wharton's Jelly Mesenchymal Stem Cells WJMSCs DMEM F12 Medium Fetal Calf Serum FBS Pen/strep L-glutamine Sterile Laminar Flow
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