Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Hydrogel Arrays Enable Increased Throughput for…
JoVE Journal
Bioengineering

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Bioengineering
Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models
 

Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

Article DOI: 10.3791/63791-v • 10:49 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1University of California Los Angeles

Capítulos

Resumo
Automatic Translation

The present protocol describes an experimental platform to assess the effects of mechanical and biochemical cues on chemotherapeutic responses of patient-derived glioblastoma cells in 3D matrix-mimetic cultures using a custom-made UV illumination device facilitating high-throughput photocrosslinking of hydrogels with tunable mechanical features.

Tags

Hydrogel Arrays Increased Throughput Screening Effects Matrix Components Therapeutics 3D Tumor Models Matrix Cues Phenotype Of Cells High-throughput Implementation Matrix Conditions Tissue-mimetic Matrices Drug Resistance New Therapeutics HEPES-buffered Solution Thiolated HA Carboxylic Acid Residues Glucuronic Acid Thiol 8arm PEG Norbornene 4arm PEG Thiol Cysteine-containing Peptide
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter