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JoVE Journal Cancer Research
Ex Vivo Organoidmodel af adenovirus-cre-medierede gendeletninger i museurtelceller
 
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Ex Vivo Organoidmodel af adenovirus-cre-medierede gendeletninger i museurtelceller

Article DOI: 10.3791/63855-v • 09:16 min • May 5th, 2022
May 5th, 2022 •
1, 1, 1, 2, 2, 2, 1,2
1Department of Urology, Roswell Park Comprehensive Cancer Center, 2Department of Pharmacology and Therapeutics, Roswell Park Comprehensive Cancer Center

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Denne protokol beskriver processen med generering og karakterisering af muse urotheliale organoider, der indeholder deletioner i gener af interesse. Metoderne omfatter høst af muse urothelceller, ex vivo-transduktion med adenovirus, der driver Cre-ekspression med en CMV-promotor, og in vitro - såvel som in vivo-karakterisering .

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Kræftforskning nummer 183
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