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Biochemistry

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JoVE Journal Biochemistry
Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
 

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Article DOI: 10.3791/63856-v • 08:31 min • July 20th, 2022
July 20th, 2022 •
*1,2, *1, *1,3, 1,4, 1,4, 5, 6, 1, 7, 8, 2, 9, 1,5,6
1Department of Biomedical Engineering, Pennsylvania State University, 2Department of Materials Science and Engineering, McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 5Materials Research Institute, Pennsylvania State University, 6Huck Institutes of the Life Sciences, Pennsylvania State University, 7Applications team, Direct Electron, 8Application Scientist, Protochips, Inc., 9Department of Biology, Wake Forest University
* These authors contributed equally

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Resumo
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Here protocols are described to prepare virus assemblies suitable for liquid-EM and cryo-EM analysis at the nanoscale using transmission electron microscopy.

Tags

High-resolution Imaging Virus Assemblies Liquid Electron Microscopy Nanoscale Visualization Flexible Structures Novel Reagents Emerging Pathogens SARS-CoV-2 Fluid Environment Dynamic Protein Visualization Vitreous Ice Antibody-based Therapies Cryo-EM Applications Molecular Dynamics Human Health And Disease TEM Liquid-EM Workflows Commercial Liquid-EM Systems Microchip Sandwich Method
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