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JoVE Journal Biochemistry
Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
 

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Article DOI: 10.3791/63891-v • 08:02 min • May 5th, 2022
May 5th, 2022 •
1, 1, 1, 1, 1
1Université Paris Cité, CNRS, Institut Jacques Monod

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We present protocols for simple actin filament microfluidic assays, in combination with fluorescence microscopy, that allow one to accurately monitor individual actin filaments in real-time while sequentially exposing them to different protein solutions.

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Microfluidics Fluorescence Microscopy Actin Filaments Actin-binding Proteins Assembly Dynamics Bundles Reactions Microfluidic Chamber PDMS Glass Cover Slip UV Exposure Air Bubbles Hot Plate
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