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Journal / Biochemistry / Self-Assembly of Microtubule Tactoids
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Biochemistry

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JoVE Journal Biochemistry
Self-Assembly of Microtubule Tactoids
 

Self-Assembly of Microtubule Tactoids

Article DOI: 10.3791/63952-v • 08:49 min • June 23rd, 2022
June 23rd, 2022 •
1, 1,2, 1, 1, 1, 1, 1, 1
1Physics Department, Syracuse University, 2Department of Molecular Biophysics and Biochemistry, Yale School of Medicine

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This article presents a protocol for the formation of microtubule assemblies in the shape of tactoids using MAP65, a plant-based microtubule crosslinker, and PEG as a crowding agent.

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Keywords: Microtubule Self-assembly Tactoids MAP65 Tubulin Rhodamine-labeled Flow Chamber Liquid Crystal Spindle-like Shapes
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