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Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions
 

Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions

Article DOI: 10.3791/64027-v • 09:54 min • July 22nd, 2022
July 22nd, 2022 •
1, 2, 1,2
1Department of Biology, Emory University, 2Department of Physics, Emory University

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This protocol describes a 96-well disruption of individual bacterially colonized Caenorhabditis elegans following cold paralysis and surface bleaching to remove external bacteria. The resulting suspension is plated on agar plates to allow accurate, medium-throughput quantification of bacterial load in large numbers of individual worms.

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Single-Worm Data Quantify Heterogeneity Caenorhabditis Elegans-Bacterial Interactions Mechanical Disruption Sea Elegans 96 Well Format Medium Throughput Quantification Bacterial Load Pasel Based Methods Larger Data Sets High Quality Single Worm Measurements Moving Parts Materials Labeled Tubes Buffers Plates Megan Taylor Research Specialist Lead Worm Sample M Nine TX Zero One Micro Centrifuge Tube Adult Worms Centrifugation Parameters M9TX01 M9 Worm Buffer External Bacteria S Medium Plus Two X Heat Killed OP50 Culture Tube
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