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JoVE Journal Immunology and Infection
Xenograft Skin Model to Manipulate Human Immune Responses In Vivo
 

Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

Article DOI: 10.3791/64040-v • 08:23 min • June 29th, 2022
June 29th, 2022 •
1, 2, 1, 1, 1, 1
1Department of Dermatology, UCSF, 2TRex Bio

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The present protocol describes how to graft human skin onto non-obese diabetic (NOD)-scid interleukin-2 gamma chain receptor (NSG) mice. A detailed description of the preparation of human skin for transplant, preparation of mice for transplant, transplantation of split-thickness human skin, and post-transplantation recovery procedure are included in the report.

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Keywords: Xenograft Skin Model Human Immune Response In Vivo Skin Transplant Skin Inflammation Dermatome Skin Grafting Mouse Model Anti-Gr1 Hair Removal
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