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Fosfolipidmediator induceret transformation i tredimensionelle kulturer
 
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Fosfolipidmediator induceret transformation i tredimensionelle kulturer

Article DOI: 10.3791/64146-v • 08:02 min • July 27th, 2022
July 27th, 2022 •
1, 1,2, 1,3, 1
1Biology Department, Indian Institute of Science Education and Research, 2Department of Biology, Center for Genomics and Systems Biology, New York University, 3Enveda Therapeutics India Pvt Ltd.

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Den nuværende protokol beskriver oprettelsen af 3D 'ovenpå ' kulturer af en ikke-transformeret brystepitelcellelinje, MCF10A, der er blevet modificeret til at studere blodpladeaktiveringsfaktor (PAF) induceret transformation. Immunfluorescens er blevet brugt til at vurdere transformationen og diskuteres detaljeret.

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Kræftforskning udgave 185 Fosfolipidmediator MCF10A-celler tredimensionelle kulturer transformation PAF
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