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Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria
 

Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria

Article DOI: 10.3791/64147-v • 11:49 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1
1Department of Medicine, Division of Nephrology, The Icahn School of Medicine at Mount Sinai

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The article describes the method for isolating conditionally immortalized glomerular endothelial cells from the kidneys of transgenic mice expressing the thermolabile simian virus 40 and photo-activatable mitochondria, PhAMexcised. We describe the procedure for glomeruli isolation from whole kidneys using beads, digestion steps, seeding, and culturing of GECs-CD31 positive.

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Isolated Conditionally Immortalized Mouse Glomerular Endothelial Cells Fluorescent Mitochondria Effect Stimuli Mitochondrial Structure In Vitro High Number Small Molecules Monotherapy Diabetic Kidney Disease Easy To Perform Specific Equipment Perfusion And Isolation Materials Kidney Glomerular Cells Hanks' Balanced Salt Solution (HBSS) 0.2-micron Filter Magnetic Beads RPMI With 10% FCS Endothelial Cell Culture Medium Six-well Plate Collagen IV Surgical Instruments
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