Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolation of Conditionally Immortalized Mouse…
JoVE Journal
Biology

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Biology
Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria
 

Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria

Article DOI: 10.3791/64147-v • 11:49 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1
1Department of Medicine, Division of Nephrology, The Icahn School of Medicine at Mount Sinai

Capítulos

Resumo
Automatic Translation

The article describes the method for isolating conditionally immortalized glomerular endothelial cells from the kidneys of transgenic mice expressing the thermolabile simian virus 40 and photo-activatable mitochondria, PhAMexcised. We describe the procedure for glomeruli isolation from whole kidneys using beads, digestion steps, seeding, and culturing of GECs-CD31 positive.

Tags

Keywords: Immortalized Glomerular Endothelial Cells Fluorescent Mitochondria In Vitro Diabetic Kidney Disease Cell Isolation Kidney Glomerular Cells Magnetic Beads Collagen IV Collagenase Type II Cell Culture
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter