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JoVE Journal Developmental Biology
En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
 

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Article DOI: 10.3791/64207-v • 08:57 min • July 27th, 2022
July 27th, 2022 •
1,2, 1,2, 1,2
1Intercellular Signalling in Cardiovascular Development and Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Ciber de Enfermedades Cardiovasculares

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Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development.

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En Face Endocardial Cushion Preparation Planar Morphogenesis Analysis Mouse Embryos Primitive Endocardium Distribution Of Endocardial Cells Anisotropy Of Other Injunctions Shape Of Single Endocardial Cell Valve Development Tissue Sections Inner Valve Regenerative Region Planar Cell Polarity Dynamics Of Endocardial Cells Intact Embryos Cardio Development Uterus Excised From Pregnant Mice Ice-cold PBT Decidua Removal Genotyping Yolk Sac Tail Digestion 4% PFA
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