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AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants
 

AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants

Article DOI: 10.3791/64428-v • 08:36 min • September 16th, 2022
September 16th, 2022 •
*1, *1, 1, 1, 1
1College of Bioscience and Biotechnology, Yangzhou University
* These authors contributed equally

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Here, we present a step-by-step protocol for performing the proximity labeling (PL) experiment in cucumber (Cucumis sativus L.) using AT4G18020 (APRR2)-AirID protein as a model. The method describes the construction of a vector, the transformation of a construct through agroinfiltration, biotin infiltration, protein extraction, and purification of biotin-labeled proteins through affinity purification technique.

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Keywords: AirID Protein-protein Interaction Proximity Labeling Cucumis Sativus Agrobacterium Tumefaciens Agroinfiltration APRR2-AirID Plant Biotechnology Molecular Biology Protein Analysis
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