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基于深度学习的冷冻电子断层扫描
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Deep Learning-Based Segmentation of Cryo-Electron Tomograms
DOI:

10:25 min

November 11, 2022

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Capítulos

  • 00:05Introduction
  • 00:45Image Import and Preprocessing
  • 02:45Creating Training Data
  • 05:13Using the Segmentation Wizard for Iterative Training
  • 06:52Apply the Network
  • 07:36Segmentation Manipulation and Cleanup
  • 08:16Results: Analysis of the Tomogram of DIV 5 Hippocampal Rat Neuron
  • 09:40Conclusion

Summary

Tadução automática

这是一种使用一个断层图的一部分作为训练输入来训练多层 U-Net 以对冷冻电子断层图进行多类分割的方法。我们描述了如何将该网络推断为其他断层图,以及如何提取分割以进行进一步分析,例如断层图平均和细丝追踪。

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