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JoVE Journal Cancer Research
Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
 

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Article DOI: 10.3791/64534-v • 07:46 min • October 11th, 2022
October 11th, 2022 •
1, 1, 1
1Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University

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Resumo
Automatic Translation

The present protocol describes establishing a murine colonic organoid system to study the activity and functioning of colonic stem cells in a claudin-7 knockout model.

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Keywords: 3D Culture Colonic Crypts Intestinal Stem Cells Ex Vivo Organoid Culture Colon Mouse Model Claudin-7 Inflammatory Bowel Disease Colorectal Cancer Crypt Isolation Epithelial Dissociation Crypt Dissociation Cell Culture
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