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Journal / Genetics / Chromatin Immunoprecipitation in the Cnidarian…
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Genetics
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JoVE Journal Genetics
Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana
 

Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana

Article DOI: 10.3791/64817-v • 11:48 min • March 17th, 2023
March 17th, 2023 •
*1, *2, 1, 2, 2, 1
1Red Sea Research Center (RSRC), Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), 2KAUST Environmental Epigenetics Program (KEEP), Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST)
* These authors contributed equally

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A chromatin immunoprecipitation (X-ChIP) assay against the histone mark H3K4me3 for the model organism Exaiptasia diaphana is presented. The specificity and effectiveness of the assay are confirmed by quantitative PCR and next-generation sequencing. This protocol enables the increased investigation of protein-DNA interactions in the sea anemone E. diaphana.

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Keywords: Chromatin Immunoprecipitation ChIP-seq Exaiptasia Diaphana Aiptasia Cnidarian Epigenetics Cross-linking Quenching Lysis Buffer Dounce Tissue Grinder Sonication
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