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JoVE Journal Biology
A Simple Protocol for Mapping the Plant Root System Architecture Traits
 

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Article DOI: 10.3791/64876-v • 11:09 min • February 10th, 2023
February 10th, 2023 •
1,2, 2,3, 1,4
1Department of Biology, Western Kentucky University, 2Plant Biotechnology Division, CSIR Central Institute of Medicinal and Aromatic Plants, 3Academy of Scientific and Innovative Research (AcSIR), 4Department of Biology, St. Joseph's University

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We use simple laboratory tools to examine the root system architecture (RSA) of Arabidopsis and Medicago. The plantlets are grown hydroponically over mesh and spread using an art brush to reveal the RSA. Images are taken using scanning or a high-resolution camera, then analyzed with ImageJ to map traits.

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Keywords: Plant Root System Architecture Non-invasive Hydroponic Method Root System Visualization Arabidopsis Seeds Surface Sterilization Polycarbonate Sheet Polypropylene Mesh MS Basal Media Hydroponics Root Growth
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