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Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis
 

Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis

Article DOI: 10.3791/64887-v • 07:56 min • May 5th, 2023
May 5th, 2023 •
1, 2, 1, 1
1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE), Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya

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The current study describes a protocol to monitor the changes in human umbilical vein endothelial cells (HUVECs) during viral infection using a real-time cell analysis (RTCA) system.

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Biology Human Umbilical Vein Endothelial Cells Viral Infection Impedance-based Real-time Cell Analysis Endothelial Cells Barrier Fluid Exchange Solute Exchange Virus Dissemination Endothelial Permeability Endothelial Cell Barrier Disruption Vascular Leakage Real-time Cell Analyzer Zika Virus (ZIKV) Infection Cell Index (CI) Transient Effects Cell Morphological Changes Vascular Integrity
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