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JoVE Journal Cancer Research
Establishment and Culture of Patient-Derived Breast Organoids
 

Establishment and Culture of Patient-Derived Breast Organoids

Article DOI: 10.3791/64889-v • 08:29 min • February 17th, 2023
February 17th, 2023 •
1,2, 1, 1, 1, 1,2
1Cold Spring Harbor Laboratory, Cold Spring Harbor, 2Genetics Graduate Program, Stony Brook University

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Resumo
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A detailed protocol is provided here for establishing human breast organoids from patient-derived breast tumor resections or normal breast tissue. The protocol provides comprehensive step-by-step instructions for culturing, freezing, and thawing human patient-derived breast organoids.

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Keywords: Patient-derived Organoids Breast Tumor Organoids 3D In Vitro Model Cellular Heterogeneity Tissue Dissociation Collagenase IV Red Blood Cell Lysis Basement Membrane Matrix AdDF+ Medium
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