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Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
 

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Article DOI: 10.3791/64917-v • 08:19 min • February 10th, 2023
February 10th, 2023 •
1,2, 1, 3, 2, 4, 1, 1
1GenPhySE, Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM, Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute

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Here, we describe a protocol to culture pig intestinal 3D organoids from cryopreserved epithelial crypts. We also describe a method to establish cell monolayers derived from 3D organoids, allowing access to the apical side of epithelial cells.

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Keywords: Pig Intestinal Epithelium Intestinal Organoids 3D Organoids Cryopreserved Crypts Epithelial Crypts 2D Monolayers Nutrient Transport Barrier Function Host-microbe Interactions Jejunum Duodenum Ileum Colon Extracellular Matrix ECM Culture Medium Passaging Dissociation DMEM Rock Inhibitor
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