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JoVE Journal Biochemistry
"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
 

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Article DOI: 10.3791/64952-v • 06:31 min • March 24th, 2023
March 24th, 2023 •
1, 1,2, 1,3,4
1Department of Laboratory Medicine, University of California, San Francisco, 2Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, 3Deptartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 4Chan Zuckerberg Biohub

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Here, we describe a proteomics workflow for characterization of the cell surface proteome of various cell types. This workflow includes cell surface protein enrichment, subsequent sample preparation, analysis using an LC-MS/MS platform, and data processing with specialized software.

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Cell Surface Capture Label-free Quantification Cell Surface Proteome Antigens Cell Membrane Proteins Mass Spectrometry-based Proteomics Tumor Cells Cancer Immunotherapies Biotin-labeled Cell Pellet Lysis Buffer Sonication Centrifugation NeutrAvidin Agarose Resin Beads Filtration Column
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