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Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
 

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Article DOI: 10.3791/65081-v 07:47 min February 10th, 2023
February 10th, 2023

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Resumo

This paper describes a method to create wounds in the epithelium of a live Clytia hemisphaerica medusa and image wound healing at a high resolution in vivo. Additionally, a technique to introduce dyes and drugs to perturb signaling processes in the epithelial cells and extracellular matrix during wound healing is presented.

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Keywords: Epithelial Wound Healing Clytia Hemisphaerica In Vivo Live Imaging Micro-wounds Scratch Wounds Microinjection Cell Migration Cell Spreading Extracellular Matrix Cell Cytoplasm
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