É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research,Southwest Medical University, 2Department of Cardiology,the Affiliated Hospital of Southwest Medical University, 3Department of Cardiology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 4Department of Pharmacology,University of Oxford
Capítulos
- 00:04Introduction
- 01:00Mouse Heart Harvest, Cannulation, and Perfusion
- 03:14Optical Mapping and Arrhythmia Induction
- 06:38Results: APD80 and CaTD80 Analysis at 10 Hz Before and After ISO Challenge
- 08:36Conclusion
Este protocolo introduz o mapeamento óptico dual-dye de corações de camundongos obtidos de animais selvagens e knock-in afetados por taquicardia ventricular polimórfica catecolaminérgica, incluindo medidas eletrofisiológicas de voltagem transmembrana e transientes intracelulares de Ca2+ com alta resolução temporal e espacial.
