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1Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research,Southwest Medical University, 2Department of Cardiology,the Affiliated Hospital of Southwest Medical University, 3Department of Cardiology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 4Department of Pharmacology,University of Oxford
Capítulos
- 00:04Introduction
- 01:00Mouse Heart Harvest, Cannulation, and Perfusion
- 03:14Optical Mapping and Arrhythmia Induction
- 06:38Results: APD80 and CaTD80 Analysis at 10 Hz Before and After ISO Challenge
- 08:36Conclusion
यह प्रोटोकॉल जंगली प्रकार से प्राप्त माउस दिलों की दोहरी डाई ऑप्टिकल मैपिंग का परिचय देता है और उच्च अस्थायी और स्थानिक संकल्प के साथ transmembrane वोल्टेज और intracellular सीए2 + यात्रियों के electrophysiological माप सहित, catecholaminergic बहुरूपी वेंट्रिकुलर tachycardia से प्रभावित जानवरों में खट्टी.
