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Journal / Biochemistry / Semi-Automated Phenotypic Analysis of Functional…
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Biochemistry

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JoVE Journal Biochemistry
Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures
 

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Article DOI: 10.3791/65086-v • 06:48 min • August 18th, 2023
August 18th, 2023 •
1, 1, 2,3, 2, 3, 1
1Department of Biochemistry, Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3CelVivo ApS

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We present a protocol for growing high-reproducible spheroids and their phenotypic characterization using image capture and proteomics.

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Keywords: 3D Cell Culture Spheroid HepG2/C3A THLE-3 Ultra-low Attachment Plate Bioreactor Proteomic Analysis LC-MS Phenotypic Analysis
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