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Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
 

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Article DOI: 10.3791/65135-v • 06:53 min • May 5th, 2023
May 5th, 2023 •
1, 1, 1
1LabPlaM - Mycoheterotrophic Plants Research Lab, Department of Plant Biology, State University of Campinas (UNICAMP)

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The current article aims to provide detailed and adequate protocols for the isolation of plant-associated endophytic fungi, long-term preservation of isolates, morphological characterization, and total DNA extraction for subsequent molecular identification and metagenomic analyses.

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Keywords: Endophytic Fungi Mycoheterotrophic Plants Fungal Isolation Fungal Characterization Total DNA Extraction PDA Culture Medium Antibiotic Supplementation Superficial Disinfection Colony Purification AA Medium Fungal Identification Fungal Subculturing
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