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JoVE Journal Biochemistry
High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
 

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

Article DOI: 10.3791/65137-v • 08:50 min • May 12th, 2023
May 12th, 2023 •
*1, *1, 1, 2,3, 2,3, 1,4
1Department of Physics, Pohang University of Science and Technology (POSTECH), 2School of Biological Sciences, Seoul National University, 3Institute for Molecular Biology and Genetics, Seoul National University, 4School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH)
* These authors contributed equally

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Here, we describe a high-speed magnetic tweezer setup that performs nanomechanical measurements on force-sensitive biomolecules at the maximum rate of 1.2 kHz. We introduce its application to DNA hairpins and SNARE complexes as model systems, but it will be also applicable to other molecules involved in mechanobiological events.

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Keywords: Magnetic Tweezers Nanomechanical Measurements Force-sensitive Elements Biomolecules Structural Changes Nucleic Acids Proteins Mechanosensitive Proteins Cardiovascular Disorders Musculoskeletal Disorders High-speed CMOS Camera Motorized Linear Stage Rotary Stepper Motor Super Luminescent Diode Piezo Lens Scanner Coverslip Preparation Pegylation
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