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Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study (Video) | JoVE
 
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Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study (Video) | JoVE

Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping

Article DOI: 10.3791/65352-v • 01:27 min • July 7th, 2023
July 7th, 2023 •
1,2, 1,2
1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University

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MRNA-anrikning klargjøring av bisulfitt-mRNA-bibliotek neste generasjons sekvensering RNA posttranskripsjonelle modifikasjoner RNA-regulering eukaryote celler RNA 5-metylcytosinmodifikasjoner RNA-metyltransferaser sykdommer kreft transkriptombrede bisulfittsekvensering cytosinmetyleringsnivåer poly (A) RNA-rensing bisulfittreaksjon bibliotekforberedelse MRNA 5-metylcytosinmodifikasjonssteder RNA-kvantitet og kvalitetsvurdering RNA-integritetsovervåking høy kvalitet Sekvens av biblioteker
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