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Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
 

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Article DOI: 10.3791/734-v • 13:03 min • April 7th, 2008
April 7th, 2008 •
1, 1, 1
1Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

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This video shows the procedure for generating induced pluripotent stem cells using inducible lentivirus that express Oct4, Sox2, c-Myc and Klf4.

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IPS Cells MEFS Forced Expression Sox-2 Oct-4 C-Myc Klf4 Pluripotency Viral Transduction Reprogramming Strategy Doxycycline-inducible Lentiviral Vectors Induced Pluripotent Stem Cells Mouse Embryonic Fibroblasts Inducible Lentiviruses Retroviral Infection Gene Expression Analysis
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