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Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
 

Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes

Article DOI: 10.3791/936-v • 08:01 min • October 16th, 2008
October 16th, 2008 •
1, 2, 2
1Department of Molecular and Cellular Physiology, Stanford University, 2Department of Molecular and Cellular Physiology, Stanford University School of Medicine

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This is intended as an introduction to patch clamp recording from Xenopus laevis oocytes. It covers vitelline membrane removal, formation of a gigaohm seal (gigaseal), and the optional conversion of the patch to the outside-out topology.

Tags

Patch Clamp Recording Ion Channels Xenopus Oocytes Electrophysiological Measurement Heterologous Cells African Clawed Frog Xenopus Laevis Macropatch Single-channel Recording Oocyte Preparation Seal Generation Successful Seal Generation
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