剖析在非人类灵长类动物脑立体定向空间
Summary
非人类灵长类动物是一种重要的翻译物种为我们理解大脑的正常处理。在人体正常和病理条件下的灵长类动物的大脑解剖组织可以提供重要的见解。
Abstract
使用非人类灵长类动物在人类发育和衰老过程的认识提供了一个优秀的翻译模式<sup> 1-6</sup>。此外，使用非人类灵长类动物最近提供的机会，自然模型复杂的精神疾病，如酗酒<sup> 7</sup>。在这里，我们描述了一种阻断冠状面vervet猴子大脑的技术（<em> Chlorocebus aethiops sabeus</em>）在立体空间中的完整的头骨。这里介绍的方法提供了一个标准节块之间和科目和块之间的最小化的部分路段平面。切片的组织块在冠状面，也有利于感兴趣的领域划分。因为单个半球的vervet猴子产量1200多节，在50μm的切片时，这种方法提供了管理大小的块。此外，通过阻断大脑切成1cm块，它有利于普及率为cyroprotection蔗糖，并允许在一个标准的低温恒温器切片块。
Protocol
第1部分：前处理的组织组织应多聚甲醛，戊二醛，或福尔马林灌注。这可以通过标准transcardial，通常用于收获其他器官灌注。在本研究的主题是深刻的盐酸氯胺酮（10毫克/公斤，IM）与戊巴比妥钠（25毫克/公斤，四）过量安乐死，镇静剂和灌注用0.1 M PBS transcardially其次，直到完全exsanguinated.This在PBS的4％多聚甲醛为5分钟（约1升）的解决方案。 第2部分：立体定?…
Discussion
圣基茨vervet（Chlorocebus aethiops sabeus）是旧世界的灵长类动物与人类类似的模式和皮层和皮层下的大脑发育率。该物种已被用来模拟复杂的人类行为障碍，如焦虑行为8，大脑半球切除术，高血压，帕金森氏病10 Alzhemier 的疾病11， 和酗酒12。最近，该物种已被用于研究自然产前乙醇暴露的神经解剖学的影响。怀孕vervets被允许在孕晚期相当于3-5每周四次的标准饮…
Disclosures
The authors have nothing to disclose.
Acknowledgements
作者想感谢他持续的技术支持Ikiel Ptito。 NSERC授予到MP。
Materials
| Material Name | Type | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| Scalpel | Fine Science Tools | 10003-12 | ||
| Scalpel blades | Fine Science Tools | 10011-00 | ||
| Scissors | Fine Science Tools | 14090-11 | Any surgical scissors are sufficient | |
| Rongeurs | Fine Science Tools | 16121-14 | ||
| Forceps | Fine Science Tools | 11027-12 | ||
| Filter paper | Fisher | 09-924-150 | ||
| Stereotaxic Frame | Kopf Instruments | |||
| Tissue slicing blade | Thomas |
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Tags
Non-human Primate BrainStereotaxic SpaceTranslational ModelDevelopmental ProcessesAging ProcessesComplex Psychiatric DisordersAlcohol AbuseCoronal PlaneVervet MonkeyIntact SkullStereotaxic SpaceStandard Plane Of SectionPartial SectionsManageable Sized BlocksHemisphereSlicingSucrose PenetrationCyroprotectionCryostat
Cite This Article
Burke, M. W., Zangenehpour, S., Boire, D., Ptito, M. Dissecting the Non-human Primate Brain in Stereotaxic Space. J. Vis. Exp. (29), e1259, doi:10.3791/1259 (2009).