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Biology
造血幹細胞の分離と移植(造血幹細胞)
Published: February 25, 2007
doi:
10.3791/157
Cristina Lo Celso
,
David Scadden
1
Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Stem Cell Institute
,
Harvard Medical School
Protocol
合計骨髄の準備四B6マウスを屠殺し、脛骨、大腿骨、股関節と背骨を取得するために解剖されています。 解剖時には、手足や骨は、PBS、2%FCS熱不活性化に保管されています(オプションの2mMのEDA – 解剖の培地を)。 きれいな骨は、解剖の培地で乳鉢と乳棒で粉砕されています。それを行うための効率的な方法は、同時に、各マウスの脛骨、大腿骨と腰、2つのスパインを…
Tags
Isolation
Transplantation
Hematopoietic Stem Cells
HSCs
check_url/157?article_type=t
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Lo Celso, C., Scadden, D. Isolation and Transplantation of Hematopoietic Stem Cells (HSCs).
J. Vis. Exp.
(2), e157, doi:10.3791/157 (2007).
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