Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

Protocol

Total bone marrow preparation Four B6 mice are sacrificed and dissected to obtain tibias, femurs, hip and spine. During the dissections, the limbs and the bones are kept in PBS 2S heat inactivated (optional 2mM EDA – dissection medium). The clean bones are crushed with mortar and pestle in dissection medium. An efficient way to do it is to crush tibias, femurs and hips of each mouse, then 2 spines at a time. The cell mixture obtained from each mouse is kept separate and filt…