光固化牙科粘合树脂安装电极或微透析探针在慢性实验中的应用
Summary
在这份报告中，我们提出了一个新的应用程序安装电极或微透析探针在慢性实验基地牙科光固化树脂。这种材料可直接粘接头盖骨。
Abstract
在慢性录音实验中，自固化牙科的丙烯酸树脂已被用来作为一个电极或微透析探针的安装基座。由于这些丙烯酸骨不债券，螺丝已被用来作为锚。然而，雀或鼠标一样的小实验动物，他们的头盖骨是很脆弱的，并不能成功举办的基石。在这份报告中，我们提出了一个新的应用程序安装电极或微透析探针在慢性实验基地牙科光固化树脂。这种材料可直接粘接头盖骨。因此，锚钉不需要手术视野，可以大大减少。以往的经验表明，粘接效果保持2个月以上。传统的树脂的窗口时，材料柔韧性和可行的几分钟。然而，这些牙齿胶粘剂的工作时间的窗口，是显着更广泛和可调。
Protocol
Discussion
引进技术相对于传统的自固化丙烯酸树脂的应用，有几个重要的优点：
1。直接键合：
可以附着到骨自固化丙烯酸一段时间，但它不会坚持到骨。因此，锚固螺丝需要修复安装基座（见图2a）。相比之下，光固化树脂可以直接让螺丝不需要骨（图2B）的债券。因此，安装基座的大小，可以大大减少。此外，光固化树脂还坚持某些金属，如果你用“金属底漆”。粘接可变材料的这一特点，使我们一个…
Disclosures
The authors have nothing to disclose.
Acknowledgements
RIKEN – BSI我们感谢我们的同事为他们的好心的支持和富有成效的意见，在此工作。我想滋森教授，博士，教授摹BOR灶火SZ表示衷心的感谢。 Kiyoji松山，卡塔琳AK缂丝和RP ð Dobolyi谁劝我在日本高级研究的研究生，研究生课程学习，微透析方法。最后，我们感谢克里斯先生Sarnecky Akitsugu高须幸雄博士在其支持视频录制。
Materials
| Material Name | Type | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| Bengalese finches | Animal | Two adult male , Body weight: 14.2 g and 14.8 g | ||
| Microdialysis probe | Tool | EICOM | total weight of probe, guide cannula, cap nut and dental adhesive resin: 0.65 to 0.78 g. | |
| Light-cured dental adhesive resin system | Kuraray Medical Inc | +Light cured composite resin (CLEARFIL AP-X). +Dual-cure dental adhesive system with the self-etching primer and bonding agent (CLEARFIL Liner Bond IIΣ). | ||
| Surgical instruments | Stereotaxic frame, surgical microscope, thermal controlled surgical pad and thermal controlled recovery chamber. Plain plugger for handling composite resin. Microspatula or sharp curette to ablate periosteum and muscles. Injection syringe and needle (30G) for inducing anesthesia. Small scissors. Fine forceps for making small craniotomy. | |||
| Light-unit | Rolence Enterprise Inc | Ultra-Lite 500E | ||
| Microscope | Konan Medical | KOM300 | with C-mount adaptor | |
| 3- CCD camera | Tool | Hitachi | HV-D30 |
Photograph 1A shows microdialysis probe (a), a cap nut (b) and a guide cannula (c). In this photo, the guide cannula is surrounded by dental resin which adheres to the skull directly. Photo B shows a bird during drug perfusion. On the top of the head, there is a microdialysis probe that is connected to a fine tefron tube. Scale bars = 1 cm for A and 2 cm for B, respectively.
Figure 1 – Photographs of a microdialysis probe (A) and a bird during drug perfusion (B)
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