श्रेणी बैक्टीरियल रोगज़नक़ों की जांच के लिए 'bioluminescent' रिपोर्टर फेज
Summary
प्राथमिकता बैक्टीरियल रोगज़नक़ों की पहचान के लिए एक सरल तरीका अनुवांशिक इंजीनियर संवाददाता फेज का उपयोग करने के लिए है. ये रिपोर्टर फेज है, जो उनके विशेष मेजबान प्रजातियों के लिए विशिष्ट हैं तेजी से bioluminescent संकेत मेजबान कोशिकाओं के जवाब transducing में सक्षम हैं. इस के साथ साथ, हम रिपोर्टर का पता लगाने के लिए फेज का उपयोग का वर्णन<em> Yersinia pestis</em>.
Abstract
Yersinia pestis and Bacillus anthracis are Category A bacterial pathogens that are the causative agents of the plague and anthrax, respectively 1. Although the natural occurrence of both diseases' is now relatively rare, the possibility of terrorist groups using these pathogens as a bioweapon is real. Because of the disease's inherent communicability, rapid clinical course, and high mortality rate, it is critical that an outbreak be detected quickly. Therefore methodologies that provide rapid detection and diagnosis are essential to ensure immediate implementation of public health measures and activation of crisis management.
Recombinant reporter phage may provide a rapid and specific approach for the detection of Y. pestis and B. anthracis. The Centers for Disease Control and Prevention currently use the classical phage lysis assays for the confirmed identification of these bacterial pathogens 2-4. These assays take advantage of naturally occurring phage which are specific and lytic for their bacterial hosts. After overnight growth of the cultivated bacterium in the presence of the specific phage, the formation of plaques (bacterial lysis) provides a positive identification of the bacterial target. Although these assays are robust, they suffer from three shortcomings: 1) they are laboratory based; 2) they require bacterial isolation and cultivation from the suspected sample, and 3) they take 24-36 h to complete. To address these issues, recombinant "light-tagged" reporter phage were genetically engineered by integrating the Vibrio harveyi luxAB genes into the genome of Y. pestis and B. anthracis specific phage 5-8. The resulting luxAB reporter phage were able to detect their specific target by rapidly (within minutes) and sensitively conferring a bioluminescent phenotype to recipient cells. Importantly, detection was obtained either with cultivated recipient cells or with mock-infected clinical specimens 7.
For demonstration purposes, here we describe the method for the phage-mediated detection of a known Y. pestis isolate using a luxAB reporter phage constructed from the CDC plague diagnostic phage ΦA1122 6,7 (Figure 1). A similar method, with minor modifications (e.g. change in growth temperature and media), may be used for the detection of B. anthracis isolates using the B. anthracis reporter phage Wβ::luxAB 8. The method describes the phage-mediated transduction of a biolumescent phenotype to cultivated Y. pestis cells which are subsequently measured using a microplate luminometer. The major advantages of this method over the traditional phage lysis assays is the ease of use, the rapid results, and the ability to test multiple samples simultaneously in a 96-well microtiter plate format.
Figure 1. Detection schematic. The phage are mixed with the sample, the phage infects the cell, luxAB are expressed, and the cell bioluminesces. Sample processing is not necessary; the phage and cells are mixed and subsequently measured for light.
Protocol
Discussion
इस विधि संवाददाता फेज करने के लिए तेजी से वाई का पता लगाने की क्षमता को दर्शाता है संवाददाता फेज के बाद pestis एक bioluminescent संकेत सुसंस्कृत वाई के जवाब transduce कर सकते हैं फेज इसके अलावा के बाद 20 मिनट के भ?…
Disclosures
The authors have nothing to disclose.
Acknowledgements
इस शोध राष्ट्रीय स्वास्थ्य संस्थान के लघु व्यापार नवाचार अनुसंधान कार्यक्रम (NIAID, 1R43AI082698-01) और खाद्य और कृषि USDA राष्ट्रीय संस्थान (NIFA, 2009-33610-20028) के द्वारा समर्थित किया गया.
Materials
| Name of the reagent | Company | Catalogue number |
|---|---|---|
| Difco LB agar, Miller | VWR | 90003-346 |
| Difco LB broth, Miller | VWR | 90003-350 |
| 17 x 100 mm culture tubes | USA Scientific | 1485-0810 |
| n-Decanal | Sigma | D7384 |
| Veritas microplate luminometer | Turner Biosystems | 9100-001 |
| Microlite microtiter 96-well plate | VWR | 62402-984 |
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