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Biology
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膜蛋白的实时测量:受体相互作用使用表面等离子体共振(SPR)
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JoVE Journal
Biology
Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
Please note that all translations are automatically generated.
Click here for the English version.
膜蛋白的实时测量:受体相互作用使用表面等离子体共振(SPR)
DOI:
10.3791/51937-v
•
09:35 min
•
November 29, 2014
•
Nurit Livnat Levanon
,
Elena Vigonsky
,
Oded Lewinson
1
Department of Biochemistry, The Bruce and Ruth Rappaport Faculty of Medicine
,
The Technion-Israel Institute of Technology
Chapters
00:05
Title
01:25
Protein Sample and Buffer Preparation
02:38
Sensor Chip and SPR Instrument Setup
03:58
Experiment Cycles
07:32
Results: On Line Monitoring of Ligand Immobilization and Determination of Kinetic Rate Constants by Injections of Multiple Analyte Concentrations
09:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
表面等离子共振(SPR)是一种无标记检测方法,实时生物分子相互作用。在这里,协议的膜蛋白:受体相互作用实验说明,在讨论该技术的优点和缺点。
Tags
Surface Plasmon Resonance
SPR
Protein-protein Interactions
Membrane Proteins
Real-time Measurements
Label-free
Kinetics
Association
Dissociation
Sensogram
ABC Transporter
Substrate Binding Protein
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