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牺牲纤维膜细胞培养生产细胞外基质纤维

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture

Please note that all translations are automatically generated. Click here for the English version.

牺牲纤维膜细胞培养生产细胞外基质纤维

DOI:

10.3791/58791-v

•

06:01 min

•

February 02, 2019

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Kevin Roberts, John Taehwan Kim, Shelby White, Jamie Hestekin, Jeffrey C. Wolchok

¹Cell & Molecular Biology Program,University of Arkansas, ²Department of Biomedical Engineering,University of Arkansas, ³Ralph E. Martin Department of Biomedical Engineering,University of Arkansas

Chapters

00:04Title
00:34Hollow Fiber Membrane (HFM) Cell Seeding and Extracellular Matrix (ECM) Extraction
03:06Extracellular Matrix Decellularization
04:25Results: Representative ECM Implant Assembly
05:21Conclusion

Summary

Automatic Translation

该方案的目标是生产针对伤口修复的整体细胞外基质纤维, 作为再生支架植入物的一部分, 适合临床前评估。这些纤维是通过在中空纤维膜上培养成纤维细胞而产生的, 并通过膜的溶解提取。

Tags

Extracellular Matrix Sacrificial Hollow Fiber Membrane Cell Culture Fibroblasts Wound Repair Biomaterial Textile Fabrication N-Methyl-2-pyrrolidone Silicone Rubber Mold

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