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牺牲纤维膜细胞培养生产细胞外基质纤维
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture
Please note that all translations are automatically generated.
Click here for the English version.
牺牲纤维膜细胞培养生产细胞外基质纤维
DOI:
10.3791/58791-v
•
06:01 min
•
February 02, 2019
•
Kevin Roberts
,
John Taehwan Kim
,
Shelby White
,
Jamie Hestekin
,
Jeffrey C. Wolchok
1
Cell & Molecular Biology Program
,
University of Arkansas
,
2
Department of Biomedical Engineering
,
University of Arkansas
,
3
Ralph E. Martin Department of Biomedical Engineering
,
University of Arkansas
Chapters
00:04
Title
00:34
Hollow Fiber Membrane (HFM) Cell Seeding and Extracellular Matrix (ECM) Extraction
03:06
Extracellular Matrix Decellularization
04:25
Results: Representative ECM Implant Assembly
05:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方案的目标是生产针对伤口修复的整体细胞外基质纤维, 作为再生支架植入物的一部分, 适合临床前评估。这些纤维是通过在中空纤维膜上培养成纤维细胞而产生的, 并通过膜的溶解提取。
Tags
Extracellular Matrix
Sacrificial Hollow Fiber Membrane
Cell Culture
Fibroblasts
Wound Repair
Biomaterial
Textile Fabrication
N-Methyl-2-pyrrolidone
Silicone Rubber Mold
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