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Chapters
- 00:00Title
- 00:48Introduction
- 01:36Preparation of hBMEC Monolayer on Petri Dishes
- 03:03Preparation of Human Hematopoietic Progenitor KG1a Cells
- 03:44Preparation of Microscope, Parallel-Plate Flow Chamber and Syringe Pump
- 06:26Capturing Leukocyte Rolling Events using NIS Elements
- 09:21Dependence of Terminal Sialylation and Surface Glycoprotein for E-selectin-Mediated KG1a Cell Rolling
- 10:43Analysis of NIS-Elements-Captured KG1a Cells and Graphing of KG1a Cell Rolling Data
- 12:27Representative Results
- 13:35Conclusion
Este relatório fornece uma representação visual de placas paralelas análise câmara de fluxo para o estudo de interações leucócitos endoteliais sob tensão de cisalhamento fisiológico. Este método é particularmente útil para investigar o papel do endotélio (E)-selectina e E-selectina leucócitos ligantes que leucócitos gatilho rolando na superfície das células endoteliais.
Tags
E-selectinLeukocyte RollingMicrovascular EndotheliumRecruitmentCytokinesLectin ReceptorCarbohydrate Counter-receptor LigandsAdhesive EventsDiapedesis ActivityLeukocyte TraffickingInflammationAnti-inflammatory TherapeuticsTechnologyParallel-plate Flow Chamber ApparatusDigital VisualizationRecording Software
