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斑马鱼的胚胎显微注射来分析基因的功能
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Microinjection of Zebrafish Embryos to Analyze Gene Function
Please note that all translations are automatically generated.
Click here for the English version.
斑马鱼的胚胎显微注射来分析基因的功能
DOI:
10.3791/1115-v
•
07:18 min
•
March 09, 2009
•
Jonathan N. Rosen
2
,
Michael F. Sweeney
,
John D. Mably
2
1
Department of Genetics
,
Harvard Medical School
,
2
Department of Cardiology
,
Children’s Hospital Boston
Chapters
00:00
Title
00:42
Introduction
01:12
Zebrafish Egg Collection
02:24
Needle Pulling, Loading, and Preparation
04:23
Injection
05:48
Representative Results: Morpholino and mRNA Injection Phenotypes
06:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该视频演示如何啉或mRNA可以注射到斑马鱼的胚胎，在单细胞阶段减少或增加特定的基因产物在随后的发展水平。
Tags
Microinjection
Zebrafish Embryos
Gene Function Analysis
Protein Manipulation
Morpholinos
Antisense Oligonucleotides
Gene Expression
MRNA Injection
Microinjection Process
Embryo Manipulation
Injection Volume
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