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收集和仓鼠卵母细胞和小鼠胚胎冷冻保存
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Biology
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Biology
Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos
Please note that all translations are automatically generated.
Click here for the English version.
收集和仓鼠卵母细胞和小鼠胚胎冷冻保存
DOI:
10.3791/1120-v
•
13:36 min
•
March 27, 2009
•
Nuno Costa-Borges
,
Sheyla González
,
Elena Ibáñez
,
Josep Santaló
1
Unitat Biologia Cellular (Facultat de Biociències)
,
Universitat Autonoma de Barcelona
Chapters
00:00
Title
00:15
Oocyte Collection
03:49
Preparation of freezing and thawing solutions
06:32
Freezing protocol
11:39
Thawing protocol
13:18
Representative results
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这个视频文章中,我们提出了一个一步一步示范如何收集和仓鼠卵母细胞冻存与解冻后的存活率。同样的程序也可以应用,成功地冻结和解冻在不同阶段的小鼠胚胎着床前的发展。
Tags
Cryopreservation
Hamster Oocytes
Mouse Embryos
Slow Freezing
Vitrification
Cryoprotectants
Ice-crystal Formation
Solution Effects
Osmotic Shock
Cryoinjuries
Programmable Freezers
Supercooling Effects
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