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在原代培养海马神经元的密集核心囊泡的实时成像
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JoVE Journal Biology
Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons
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在原代培养海马神经元的密集核心囊泡的实时成像

DOI:
10.3791/1144-v

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09:45 min

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May 29, 2009

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1Department of Biological Sciences,Simon Fraser University

Chapters

  • 00:05Title
  • 00:54Introduction
  • 01:27Transfection of Neurons Using Lipofectamine 2000
  • 04:09Live Imaging of GFP-Tagged Dense-Core Vesicles in Cultured Hippocampal Neurons
  • 08:25Representative Results
  • 09:12Conclusion

Summary

Automatic Translation

Automatic Translation

活细胞成像研究细胞器官贩卖的动态时，特别实用。在这里，我们描述了一个致密核心囊泡在培养的神经元，采用宽视场荧光显微镜的实时成像的协议。此协议是灵活的和可适应图像的其它细胞器如线粒体，内体，和过氧化物酶。

Tags

Live ImagingDense-core VesiclesPrimary Cultured Hippocampal NeuronsFluorescent ProbesGreen Fluorescent Protein (GFP)Cell BiologyDynamic Cellular ProcessesIntracellular CompartmentsCellular StructuresCytoskeletonOrganelle TransportMembrane DynamicsTechnical ChallengesPost-mitotic NeuronsPhototoxicityPhotobleachingCell HealthLipid-based Transfection MethodTransfection RatePolarized NeuronsAxonsDendritesDirectionality Of Transport

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