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初压雌核发育二倍体的斑马鱼
JoVE Journal
Biology
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JoVE Journal Biology
Making Gynogenetic Diploid Zebrafish by Early Pressure
DOI:

12:40 min

June 30, 2009

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Chapters

  • 00:05Title
  • 00:07Introduction
  • 01:43Preparing for Sperm Collection
  • 02:50Sperm Collection
  • 04:19UV Inactivation of Sperm
  • 05:33Egg Collection and in vitro Fertilization
  • 07:16Early Pressure
  • 09:56Representative Results: 24 Hour Haploids and Diploids
  • 11:56Conclusion

Summary

Automatic Translation

这是一个产生阻塞后,立即用紫外线灭活精子受精的第二次减数分裂雌核发育二倍体斑马鱼的胚胎(仅来自母亲的遗传贡献的胚胎)的方法。 EP胚胎是不完全纯合的,由于在第一次减数分裂重组,但他们是在所有尚未从他们的着丝粒分离,通过重组位点纯合子。

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