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Journal
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游离成年哺乳动物元DNA的细胞核内内微量注射
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons
Please note that all translations are automatically generated.
Click here for the English version.
游离成年哺乳动物元DNA的细胞核内内微量注射
DOI:
10.3791/1614-v
•
13:39 min
•
December 10, 2009
•
Van B. Lu
,
Damian J. Williams
,
Yu-Jin Won
,
Stephen R. Ikeda
1
Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism (NIAAA)
,
National Institutes of Health (NIH)
Chapters
00:00
Title
00:55
Introduction
01:39
Preparation for Injection and Fabrication of Injection Pipettes
04:54
Intranuclear Microinjection
12:00
Whole-cell Recordings of Currents through Heterologously-Expressed G-protein Coupled Inwardly Rectifying K+ (GIRK) Channels from SCG Neurons
12:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
核内基因直接注射是有效的有丝分裂后细胞转染技术。此方法提供从单个或多个基因结构的外源蛋白的表达水平高,使蛋白质的功能与生理有关环境的各种单细胞分析研究。
Tags
Intranuclear Microinjection
DNA
Dissociated Adult Mammalian Neurons
Protein Function
Primary Neuronal Cell Cultures
Heterologous Protein Expression
Electroporation
Chemically-mediated Transfection
CDNA Constructs
Adult Sympathetic Neurons
Inverted Microscope
Glass Injection Pipet
N2(g) Pressure Delivery System
Micromanipulator
Transfection Methods
Single Cell Studies
Electrophysiology
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