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Chapters
- 00:00Title
- 00:42Introduction
- 01:17Pool Design and Oligo Recovery
- 03:53Co-immunoprecipitation of the Oligo Pool with an RNP of Interest
- 07:50Labeling the Samples and Hybridizations to the Microarray
- 10:50Mapping RNPs on Pre-mRNAs Representative Results
- 11:56Conclusion
A causa della natura transitoria del pre-mRNA, può essere difficile da isolare e studiare<em> In vivo</em>. Qui, vi presentiamo un romanzo<em> In vitro</emApproccio> per indagare le interazioni RNA-proteina con un pool di sintesi oligo che le piastrelle in regioni selezionate del pre-mRNA.
Tags
Rapid High-throughput MethodMapping RibonucleoproteinsRNPsHuman Pre-mRNACo-immunoprecipitateRNA Binding ProteinsSplicing FactorIn Vitro ApproachOligonucleotide PoolMolecular SelectionTwo Color Array StrategyEnrichmentHigh-resolution MapsSequence-specific DeterminatesStructural DeterminatesRibonucleoprotein BindingUnique AdvantageSampling BiasIP TechniquesSELEX TechniquesFlexibility
