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Bloemen-dip Transformatie van Arabidopsis thaliana Om te onderzoeken PTSO2:: β-glucuronidase Reporter Gene Expression
JoVE Journal
Biology
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JoVE Journal Biology
Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
DOI:

10:24 min

June 11, 2010

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Chapters

  • 00:00Title
  • 01:00Introduction
  • 01:35Floral-dip Transformation of Arabidopsis thaliana
  • 05:20Selecting for Transgenic Plants
  • 07:25Examining pTS02::GUS Expression Patterns
  • 09:26Representative Images of GUS Expression in Arabidopsis thaliana Transformants
  • 09:57Conclusion

Summary

Automatic Translation

Dit artikel illustreert de bloemen-dip methode van<em> Agrobacterium tumefaciens</em>-Gemedieerde transformatie van<em> Arabidopsis thaliana</em>. Door de introductie van een cel-cyclus gereguleerde promotor-reporter,<em> PTSO2:: β-glucuronidase (GUS)</em>, In<em> Arabidopsis</em>, We zien hoe een verslaggever GUS expressie in transgene zaailingen detecteert.

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