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Journal
/
Biology
/
Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation
/
Ultrathin Sectioning for Epon Post-Embedding Correlative Light and Electron Microscopy (CLEM)
JoVE Journal
Biology
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JoVE Journal
Biology
Ultrathin Sectioning for Epon Post-Embedding Correlative Light and Electron Microscopy (CLEM)
Ultrathin Sectioning for Epon Post-Embedding Correlative Light and Electron Microscopy (CLEM)
Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation
DOI:
10.3791/201225-v
•
00:53 min
•
January 12, 2024
•
Shuyuan Wang*
1
,
Haiyan Xiong*
1,2
,
Qiyuan Chang*
1,3
,
Xudong Zhuang*
4,5,6
,
Yaochen Wu
3
,
Xinrui Wang
5,6
,
Congxian Wu
,
Zhifei Fu
7,8
1
Public Technology Service Center
,
Fujian Medical University
,
2
The School of Pharmacy
,
Fujian Medical University
,
3
The School of Basic Medical Sciences
,
Fujian Medical University
,
4
College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics
,
Fujian Medical University
,
5
Medical Research Center
,
Fujian Maternity and Child Health Hospital
,
6
NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate
,
Fujian Maternity and Child Health Hospital
,
7
Institute of Neuroscience
,
Fujian Medical University
,
8
Fujian Key Laboratory of Molecular Neurology
,
Fujian Medical University
Tags
Ultrathin Sectioning
Epon Post-embedding
Correlative Light And Electron Microscopy
CLEM
Diamond Knife
Ultra Microtome
100 Nanometers
Water Bath
Gold Nanoparticle Coated Cover Glass
Sample Loop
Marker Pen
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