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Image Analysis of Brightfield and Fluorescence Images of the Mouse Brain Sections for Epon Post-Embedding Correlative Light and Electron Microscopy
 

Image Analysis of Brightfield and Fluorescence Images of the Mouse Brain Sections for Epon Post-Embedding Correlative Light and Electron Microscopy

Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

Article DOI: 10.3791/201227-v • 02:13 min • January 12th, 2024
January 12th, 2024 •
*1, *1,2, *1,3, *4,5,6, 1,3, 4,5,6, 1, 1,7,8
1Public Technology Service Center, Fujian Medical University, 2The School of Pharmacy, Fujian Medical University, 3The School of Basic Medical Sciences, Fujian Medical University, 4College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, 5Medical Research Center, Fujian Maternity and Child Health Hospital, 6NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate, Fujian Maternity and Child Health Hospital, 7Institute of Neuroscience, Fujian Medical University, 8Fujian Key Laboratory of Molecular Neurology, Fujian Medical University
* These authors contributed equally

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Keywords: Correlative Light And Electron Microscopy CLEM Fluorescence Microscopy Electron Microscopy Ultrastructure Epon Embedding MEosEM Image Alignment Image Registration
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