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Journal
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Biology
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Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation
/
إنشاء خريطة الملاحة ل CLEM وإعداد المقاطع فائقة النحافة للمجهر الإلكتروني
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JoVE Journal
Biology
Creating the Navigation Map for CLEM and Preparation of the Ultrathin Sections for Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
إنشاء خريطة الملاحة ل CLEM وإعداد المقاطع فائقة النحافة للمجهر الإلكتروني
Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation
DOI:
10.3791/201226-v
•
02:38 min
•
January 12, 2024
•
Shuyuan Wang*
1
,
Haiyan Xiong*
1,2
,
Qiyuan Chang*
1,3
,
Xudong Zhuang*
4,5,6
,
Yaochen Wu
3
,
Xinrui Wang
5,6
,
Congxian Wu
,
Zhifei Fu
7,8
1
Public Technology Service Center
,
Fujian Medical University
,
2
The School of Pharmacy
,
Fujian Medical University
,
3
The School of Basic Medical Sciences
,
Fujian Medical University
,
4
College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics
,
Fujian Medical University
,
5
Medical Research Center
,
Fujian Maternity and Child Health Hospital
,
6
NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate
,
Fujian Maternity and Child Health Hospital
,
7
Institute of Neuroscience
,
Fujian Medical University
,
8
Fujian Key Laboratory of Molecular Neurology
,
Fujian Medical University
Tags
Navigation Map
Ultrathin Sections
Electron Microscopy
MScarlet
Annulus Marker
Bright Field Imaging
Fluorescent Imaging
Stitching
Cells Of Interest
Fluorescent Images
Bright Field Images
Mounting Buffer
Double Distilled Water
Pioloform Film
Slot Grid
Paraform
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