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Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation
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Análise de Imagens de Campo Claro e Fluorescência das Seções Cerebrais de Camundongos para Epon Microscopia Correlativa de Luz e Eletrônica Pós-Incorporação
JoVE Journal
Biology
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JoVE Journal Biology
Image Analysis of Brightfield and Fluorescence Images of the Mouse Brain Sections for Epon Post-Embedding Correlative Light and Electron Microscopy

Análise de Imagens de Campo Claro e Fluorescência das Seções Cerebrais de Camundongos para Epon Microscopia Correlativa de Luz e Eletrônica Pós-Incorporação

Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

DOI:
10.3791/201227-v

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02:13 min

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January 12, 2024

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, , , , , , ,
1Public Technology Service Center,Fujian Medical University, 2The School of Pharmacy,Fujian Medical University, 3The School of Basic Medical Sciences,Fujian Medical University, 4College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, 5Medical Research Center,Fujian Maternity and Child Health Hospital, 6NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate,Fujian Maternity and Child Health Hospital, 7Institute of Neuroscience,Fujian Medical University, 8Fujian Key Laboratory of Molecular Neurology,Fujian Medical University

Tags

Image AnalysisBrightfieldFluorescenceMouse BrainEpon Post-embeddingCorrelative Light And Electron MicroscopyMScarletImageJStitchingSum Intensity ProjectionGold NanoparticlesMerge ChannelsRGB Color

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