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Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

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Registrering af fluorescensmikroskopibilledet med elektronmikroskopibilledet for korrelativt lys og elektronmikroskopi

JoVE Journal
Biology

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JoVE Journal Biology

Registration of the Fluorescence Microscopy Image with the Electron Microscopy Image for Correlative Light and Electron Microscopy

Registrering af fluorescensmikroskopibilledet med elektronmikroskopibilledet for korrelativt lys og elektronmikroskopi

Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

DOI:

10.3791/201228-v

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02:30 min

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January 12, 2024

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Shuyuan Wang*¹, Haiyan Xiong*^1,2, Qiyuan Chang*^1,3, Xudong Zhuang*^4,5,6, Yaochen Wu³, Xinrui Wang^5,6, Congxian Wu, Zhifei Fu^7,8

¹Public Technology Service Center,Fujian Medical University, ²The School of Pharmacy,Fujian Medical University, ³The School of Basic Medical Sciences,Fujian Medical University, ⁴College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁵Medical Research Center,Fujian Maternity and Child Health Hospital, ⁶NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate,Fujian Maternity and Child Health Hospital, ⁷Institute of Neuroscience,Fujian Medical University, ⁸Fujian Key Laboratory of Molecular Neurology,Fujian Medical University

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Keywords: Fluorescence Microscopy Electron Microscopy Correlative Light And Electron Microscopy Image Registration EasyCLIMv0 Non-rigid Alignment Gold Nanoparticles ImageJ Image Processing Composite Image Image Channels

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